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(a) Harvest EC stem cells with 1 mL of trypsin/EDTA solution to produce a single-cell suspension and determine cell number as described above. (b) To induce cell differentiation, seed cells at 5 105 cells per sterile 9-cm Petri dish in 10 mL of growth medium. (c) Maintain suspension cultures for 24 h before adding retinoic acid to a nal concentration of 10 M. (d) Incubate cells for a further 2 weeks, changing the growth medium supplemented with retinoic acid every 3 4 days. Suspended aggregations of cells readily form during the rst week of culture (Fig. 6.9). It is necessary to pellet aggregates by gentle centrifugation at 20 g for 2 min before resuspending cells in fresh growth medium. (e) Transfer cell suspension to a 15-mL centrifuge tube and gently pellet cell aggregates at 20 g for 2 min. Resuspend in fresh growth medium without retinoic acid and seed onto substrates coated in poly-L-ornithine (10 g/mL) and laminin (10 g/mL).

This chapter gave you a whirlwind tour of the remarkable formatting capa bilities offered by the table and frame tag sets. From the basics of the table, tr, and td tags, you learned about the many different attributes of these tags and how they can work together to produce quite sophisticated and interesting layouts. In addition, the exploration of frames offered a new way of looking at site design, particularly in terms of navigational control. I introduce some tricky formatting tag sets, so make sure you ve had a chance to digest these before you proceed. 10 introduces a bunch of advanced design features, including changing backgrounds, Exploreronly marquees, and lots more!

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Equation (9-41) can be rearranged to give Po N2 D5 2 C = y T3 1.36 (9-42)

27 Using Dreamweaver Templates 28 Using Repeating Elements 29 Maximizing Browser Targeting 30 Building Web Sites with a Team 31 Integrating with XML

and these may be solved independently for q5 and A. A procedure such as this is termed fixing the gauge. The particular choice of gauge defined by (2.116) is called the Lorentz gauge [more precisely, the Lorentz family of gauges; see Jackson (1975), 56.51, and (2.116) is termed the Lorentz condition. The Lorentz gauge is widely used because it leads to the two decoupled wave equations derived above, and because equations in the Lorentz gauge are easily written in covariant form. However, other gauges are useful for certain purposes. The Coulomb gauge,

Getting FileMaker Working for You ....................................................................................35 Starting and exiting from FileMaker ..........................................................................36 Creating, saving, and closing files ..............................................................................37 Handling files and data safely ....................................................................................38 Earlier file formats and conversion issues ..................................................................40 Finding Your Way Around ..................................................................................................41 The modes and their uses ..........................................................................................43 Navigating and viewing data .....................................................................................43 Searching and the FileMaker Find/Omit puzzle .........................................................45 Screen elements and what they re for ........................................................................47 Entering and Editing Data ...................................................................................................48 Creating and duplicating records ..............................................................................48 Field definitions: Validation and dependencies .........................................................49 The significance of commitment................................................................................49 The Ins and Outs ................................................................................................................50 Importing and exporting data....................................................................................51 Previewing and printing options ...............................................................................52 Send/Save as PDF and Excel ......................................................................................53 Getting to Know the Relatives .............................................................................................54 Ways to view and edit related data ............................................................................54 The importance of context ........................................................................................55 Making complexity simple in practice .......................................................................55 Optimizing the Application .................................................................................................55 Preference settings for your workstation ....................................................................56 File options for the current database .........................................................................59

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Left-click the Row Labels or Column Labels field in the Report Layout area and choose Label Filters from the pop-up menu. This brings up another submenu where you can choose from a list of available operators (see Table 10-1). Right-click an item in the Row Labels or Column Labels area and choose Filter Label Filters from the pop-up menu. Left-click a field in the Fields section of the PivotTable Field List dialog box and choose Label Filters from the pop-up menu. This brings up another submenu where you can choose from a list of available operators (see Table 10-1).